EasyRoti PRO 1500ml Rotifer Culture Gel
EasyRoti PRO from Easy Reefs is a 1500ml bulk pouch of concentrated phytoplankton for rotifer cultures. Comprised of the two main microalgae species at proper concentration of Nannochloropsis (80%) and Tetraselmis (20%).
Easybooster Composition:
Nannochloropsis (80%) / Tetraselmis (20%) The special characteristics of each one of the micro algae in this formula provide an optimum nutritional profile for rotifers.
Dosage:
The dosage will depend on the number of rotifers reached. The average consumption is of 4 ml EasyRoti per litre of rotifer culture per day (25% of rotifer will be harvested every day).
Instructions for use:
To keep a rotifer culture follow the directions below:
Material needed:
- 3 cylinder flasks of 2 L with slow bubbling. Rotifer inoculums.
- 50 micron net
Food needed:
Easy Reefs EasyRoti or Nannochloropsis (80%) +Tetraselmis (20%)
Culture cycle from strain: 5 days
Recommended sea water density: 1019, although they can be cultured well up to 1022.
Culture temperature: 22ºC to 28ºC. Optimum culture temperature: 24ºC.
To filter the rotifer you should use a 50 micron net.
Once you start the culture you will be able to harvest rotifers after 6 days. You will have one flask for the “strain” and another one for the “production” culture.
DAY 1
Initial conditions: “Strain” culture with 500 mL of sea water, number of rotifers 400 rot./mL to 600 rot./mL and 4 ml of Easyroti Nannochloropsis (80%) +Tetraselmis (20%).
DAY 2
Initial conditions: 1 L of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 500 rot/mL
DAY 3
Initial conditions: 1.4 L of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 500-600 rot/mL
DAY 4
Initial conditions: 1.8 L of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 600 rot/mL
DAY 5
Initial conditions: 2 L of rotifer culture. It will be nearly clear and with no microalgae. Estimated rotifer number: 700-800 rot/mL
DAY 6
Initial conditions: “Strain” culture the same as day 2. “Production” culture 2 L with 800 rot/mL, it will be nearly clear and with no microalgae.